The PNPLA5 Polyclonal Antibody (PACO58248) is a valuable tool for research involving PNPLA5, a member of the patatin-like phospholipase domain-containing protein family. The antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By targeting the PNPLA5 protein, this antibody allows for the detection and analysis of PNPLA5 expression in various cell types, making it an essential tool for studies in lipid metabolism and liver function.PNPLA5 plays a crucial role in lipid metabolism, specifically in triglyceride hydrolysis, making it a key player in the regulation of lipid storage and energy homeostasis. Dysregulation of PNPLA5 has been implicated in various metabolic disorders, including non-alcoholic fatty liver disease and obesity.
Research into the function and regulation of PNPLA5 is critical for understanding the mechanisms underlying these conditions and identifying potential therapeutic targets.In summary, the PNPLA5 Polyclonal Antibody (PACO58248) is a highly specific and reliable tool for studying PNPLA5 expression and function in a variety of research applications, particularly in the fields of lipid metabolism, liver biology, and metabolic diseases. Its use can provide valuable insights into the role of PNPLA5 in health and disease, ultimately paving the way for the development of novel therapeutic interventions.
Western Blot. Positive WB detected in: Colo320 whole cell lysate, A549 whole cell lysate. All lanes: PNPLA5 antibody at 3.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 36 kDa. Observed band size: 48 kDa.
IHC image of PACO58248 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO58248 diluted at 1:500 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PNPLA5: Lipid hydrolase. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.1.1.-Chromosomal Location of Human Ortholog: 22q13.31Cellular Component: cytosolMolecular Function: triacylglycerol lipase activityBiological Process: cellular lipid metabolic process
UniProt Protein Details:
NCBI Summary:
This gene is a member of the patatin-like phospholipase family; its encoded protein has been shown to inhibit transacylation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]
