The PNPLA2 Polyclonal Antibody (CAB6245) is a valuable tool for research involving PNPLA2, a key enzyme involved in lipid metabolism and energy homeostasis. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the PNPLA2 protein, this antibody allows for the detection and analysis of PNPLA2 expression in various cell types, making it ideal for studies in metabolic disorders and obesity research.
PNPLA2, also known as adipose triglyceride lipase (ATGL), plays a crucial role in lipolysis, the breakdown of stored fats into energy. Dysregulation of PNPLA2 has been linked to metabolic diseases such as obesity, diabetes, and fatty liver disease, making it a promising target for therapeutic interventions. Understanding the function of PNPLA2 is essential for developing effective strategies to combat these prevalent health issues by targeting lipid metabolism pathways.
Product Name:
ATGL/PNPLA2 Rabbit Polyclonal Antibody
SKU:
CAB6245
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 45-257 of human PNPLA2 (NP_065109.1).
Cell membrane, Lipid droplet, Single-pass type II membrane protein.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.
Purification Method:
Affinity purification
Gene ID:
57104
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit pAb (CAB6245) at 1:500 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 60s.
