The PNPLA1 Polyclonal Antibody (PAC039106) is a valuable tool for research involving PNPLA1, a protein involved in lipid metabolism and skin barrier function. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the PNPLA1 protein, researchers can effectively detect and analyze its expression in various cell types, making it an ideal choice for studies in dermatology and lipid metabolism research.
PNPLA1, also known as Patatin-like phospholipase domain-containing protein 1, plays a crucial role in lipid metabolism in the skin and is essential for maintaining the skin barrier function. Dysregulation of PNPLA1 has been implicated in various skin disorders, making it a promising target for therapeutic interventions. Understanding the function of PNPLA1 is key to developing treatments for conditions like ichthyosis and other skin diseases associated with lipid metabolism dysfunction.
Western Blot. All lanes: PNPLA1 antibody at 12µg/ml. Lane 1: Mouse spleen tissue. Lane 2: Mouse skeletal muscle tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 48, 49 kDa. Observed band size: 58 kDa.
Immunofluorescent analysis of HepG2 cells using PACO39106 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO39106 at dilution of 1:100.
Background:
Lipid hydrolase. Important in the formation of the epidermal lipid barrier. Plays a role in glycerophospholipid metabolism.
Synonyms:
Patatin-like phospholipase domain-containing protein 1 (EC 3.1.1.-), PNPLA1
UniProt Protein Function:
PNPLA1: Lipid hydrolase. 3 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.1.-; HydrolaseChromosomal Location of Human Ortholog: 6p21.31Cellular Component: cytoplasmDisease: Ichthyosis, Congenital, Autosomal Recessive 10
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the patatin-like phospholipase (PNPLA) family, which is characterized by the presence of a highly conserved patatin domain. PNPLA family members have diverse lipolytic and acyltransferase activities, and are key elements in lipid metabolism. While other members of this family have been well characterized, the function of this gene remained an enigma. However, recent studies show that this gene is expressed in the skin epidermal keratinocytes, and has a role in glycerophospholipid metabolism in the cutaneous barrier. Consistent with these observations, mutations in this gene are associated with ichthyosis in human (autosomal recessive congenital ichthyoses, ARCI) and dog. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
