The PNP Polyclonal Antibody (CAB14032) is a valuable tool for researchers studying PNP, a key enzyme involved in purine metabolism. Purine nucleoside phosphorylase (PNP) plays a crucial role in the salvage pathway of purine metabolism, catalyzing the conversion of purine nucleosides to their respective bases. The antibody, raised in rabbits, is highly specific to human PNP and has been validated for use in various applications, including Western blot and immunohistochemistry.PNP is essential for maintaining cellular purine levels and nucleotide synthesis, making it a target of interest in cancer research and other diseases related to purine metabolism dysregulation.
By detecting and analyzing PNP expression in different cell types and tissues, researchers can gain insights into the role of this enzyme in normal physiological processes and disease pathogenesis. This antibody provides a reliable tool for studying PNP function and regulation, contributing to advancements in the understanding and treatment of conditions associated with purine metabolism abnormalities.
Product Name:
PNP Rabbit Polyclonal Antibody
SKU:
CAB14032
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2).
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
Purification Method:
Affinity purification
Gene ID:
4860
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PNP antibody (CAB14032) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
