The PNMT Polyclonal Antibody (CAB5553) is a valuable tool for researchers studying PNMT, an enzyme involved in the biosynthesis of the neurotransmitter norepinephrine. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to PNMT, this antibody allows for the detection and analysis of PNMT levels in various cell types, making it an essential component for studies in neuroscience and cardiovascular research.
PNMT plays a crucial role in regulating the synthesis of norepinephrine, a neurotransmitter that plays a key role in the body's stress response and cardiovascular function. Dysregulation of PNMT activity has been linked to various neurological and cardiovascular disorders, making it a promising target for drug development and therapeutic interventions. Understanding the function of PNMT is essential for advancing our knowledge of these conditions and developing effective treatments.
Product Name:
PNMT Rabbit Polyclonal Antibody
SKU:
CAB5553
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human PNMT (NP_002677.1).
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5409
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PNMT antibody (CAB5553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
