The PNKP Polyclonal Antibody (CAB6693) is a valuable tool for researchers studying the PNKP protein, an essential enzyme involved in DNA repair and maintenance. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PNKP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for investigations in molecular biology and genetics.
PNKP, also known as polynucleotide kinase 3'-phosphatase, plays a crucial role in repairing DNA damage caused by various factors, including radiation and oxidative stress. Dysregulation of PNKP activity has been implicated in diseases such as neurodegenerative disorders and cancer, making it a promising target for therapeutic interventions. Understanding the function of PNKP is essential for developing strategies to combat DNA damage-related diseases and improve patient outcomes.
Product Name:
PNKP Rabbit Polyclonal Antibody
SKU:
CAB6693
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Purification Method:
Affinity purification
Gene ID:
11284
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PNKP Rabbit pAb (CAB6693) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
