The PMS2 Rabbit Monoclonal Antibody (CAB4577) is a highly specific antibody designed for research involving the PMS2 protein, a key component of the DNA mismatch repair system. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, particularly in Western blotting.The PMS2 protein is essential for ensuring the fidelity of DNA replication and repair processes, and mutations in the gene encoding PMS2 have been associated with Lynch syndrome, a hereditary cancer disorder. The ability of this antibody to bind specifically to PMS2 allows for the precise detection and analysis of this important protein in diverse cell types, making it an invaluable tool for studies in cancer research and genetic diseases.
Understanding the role of PMS2 in DNA repair mechanisms and its implications for cancer susceptibility is crucial for the development of targeted therapies and precision medicine approaches. By utilizing the PMS2 Rabbit Monoclonal Antibody (CAB4577), researchers can further unravel the intricate molecular pathways involving PMS2 and contribute to advancements in the field of personalized medicine.
Product Name:
PMS2 Rabbit Monoclonal Antibody
SKU:
CAB4577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PMS2 (P54278).
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.
Purification Method:
Affinity purification
Gene ID:
5395
Clone Number:
ARC1039
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PMS2 Rabbit mAb (CAB4577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
