The PMS2 Polyclonal Antibody is a valuable tool for researchers studying the PMS2 protein, a key player in DNA mismatch repair and maintaining genomic stability. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PMS2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in genetics and cancer research.
PMS2 is a crucial component of the DNA mismatch repair system, responsible for correcting errors that occur during DNA replication. Dysregulation of PMS2 function has been linked to increased mutation rates and the development of various cancers, making it an attractive target for therapeutic interventions. By elucidating the role of PMS2 in genomic stability, researchers can gain valuable insights into the mechanisms underlying cancer development and potentially identify novel treatment pathways.
Product Name:
PMS2 Polyclonal Antibody
SKU:
CAB21547
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526.2).
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.
Purification Method:
Affinity purification
Gene ID:
5395
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from wild type(WT) and PMS2 knockout (KO) HeLa(KO) cells, using PMS2 antibody (CAB21547) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
