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PMS2 Polyclonal Antibody (CAB21547)

SKU:
CAB21547
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
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Description

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Product Name:PMS2 Polyclonal Antibody
SKU:CAB21547
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526.2).
Sequence:ADLE KPMV EKQD QSPS LRTG EEKK DVSI SRLR EAFS LRHT TENK PHSP KTPE PRRS PLGQ KRGM LSSS TSGA ISDK GVLR PQKE AVSS SHGP SDPT DRAE VEKD SGHG STSV DSEG FSIP DTGS HCSS EYAA SSPG DRGS QEHV DSQE KAPK TDDS FSDV DCHS NQED TGCK FRVL PQPT NLAT PNTK RFKK EEIL SSSD ICQK LVNT QDMS ASQV DVAV KINK KVVP LDFS MSSL AKRI KQLH HEAQ QSEG EQNY RKFR AKIC PGEN QAAE DELR KEIS K
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:MLH4; PMS-2; PMSL2; HNPCC4; LYNCH4; MMRCS4; PMS2CL; S2
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:96kDa
Observed MW:110kDa

The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.

Purification Method:Affinity purification
Gene ID:5395
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from wild type(WT) and PMS2 knockout (KO) HeLa(KO) cells, using PMS2 antibody (CAB21547) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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