The PMS2 Monoclonal Antibody is a highly specific tool for researchers studying PMS2, a key protein involved in DNA mismatch repair. This antibody, produced using hybridoma technology, has been validated for use in various applications, including immunofluorescence and flow cytometry. It recognizes the PMS2 protein in human samples, allowing for accurate detection and analysis in a variety of experimental settings.PMS2 plays a critical role in maintaining genomic stability by correcting errors that occur during DNA replication. Mutations in the PMS2 gene are associated with Lynch syndrome, a hereditary cancer syndrome characterized by a high risk of developing colorectal and other types of cancer.
Research on PMS2 and its functions is crucial for understanding the molecular mechanisms underlying cancer development and progression.With its high specificity and sensitivity, the PMS2 Monoclonal Antibody is a valuable tool for investigating the role of PMS2 in DNA repair pathways and its implications for cancer biology. Researchers in the fields of oncology, genetics, and molecular biology will find this antibody indispensable for their studies on genomic integrity and cancer susceptibility.
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.
Purification Method:
Affinity purification
Gene ID:
5395
Clone Number:
ARC2479
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using [KO Validated] PMS2 Rabbit mAb (CAB22350) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
