The PML-RARA Polyclonal Antibody (CAB7525) is specifically designed for research involving the PML-RARA fusion protein, a key biomarker in acute promyelocytic leukemia (APL). The antibody, produced in rabbits, is highly reactive with human samples and is validated for use in Western blot and immunohistochemistry applications. It binds specifically to the PML-RARA fusion protein, enabling accurate detection and analysis in various cell types, making it an essential tool for studies in cancer research and diagnostics.The PML-RARA fusion protein is a result of a genetic translocation seen in the majority of APL cases, making it a critical target for diagnosis and monitoring of the disease.
Understanding the expression and activity of PML-RARA is crucial for the development of targeted therapies and personalized medicine approaches for APL patients. The PML-RARA Polyclonal Antibody provides researchers with a reliable tool to study the fusion protein's role in the pathogenesis of APL and to facilitate the development of novel treatment strategies.
Product Name:
PML/RARA Rabbit Polyclonal Antibody
SKU:
CAB7525
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 465-664 of human PML/RARα.
Promyelocytic leukemia/retinoic acid receptor alpha or PML-RARA refers to an abnormal fusion gene sequence. It is a specific rearrangement of genetic material from two separate chromosomes (chromosomal translocation) and is associated with a specific type of leukemia.Promyelocytic leukemia (PML) is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Retinoic acid receptor alpha(RARA), regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia.
Purification Method:
Affinity purification
Gene ID:
5371/5914
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cells, using PML/RARA antibody (CAB7525) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
