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[KO Validated] PML Rabbit Polyclonal Antibody (CAB1184)

SKU:
CAB1184
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • KO Validated Antibodies 1 Anti-PML Antibody CAB1184KO Validated
  • KO Validated Antibodies 1 Anti-PML Antibody CAB1184KO Validated
  • KO Validated Antibodies 1 Anti-PML Antibody CAB1184KO Validated
  • KO Validated Antibodies 1 Anti-PML Antibody CAB1184KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PML Rabbit Polyclonal Antibody
SKU:CAB1184
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150241.2).
Sequence:AVDA RYQR DYEE MASR LGRL DAVL QRIR TGSA LVQR MKCY ASDQ EVLD MHGF LRQA LCRL RQEE PQSL QAAV RTDG FDEF KVRL QDLS SCIT QGKD AAVS KKAS PEAA STPR DPID VDLP EEAE RVKA QVQA LGLA EAQP MAVV QSVP GAHP VPVY AFSI KGPS YGED VSNT TTAQ KRKC SQTQ CPRK VIKM ESEE GKEA RLAR SSPE QPRP STSK AVSP PHLD GPPS PRSP VIGS EVFL PNSN HVAS GAGE AEER VVVI SSSE DSDA ENSS SREL DDSS SESS DLQL EGPS TLRV LDEN L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:MYL; RNF71; PP8675; TRIM19; ML
Positive Sample:DU145
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasmic side, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, PML body, Peripheral membrane protein, nucleolus, nucleoplasm.
Calculated MW:98kDa
Observed MW:70-130kDa

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Purification Method:Affinity purification
Gene ID:5371
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (CAB1184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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