The Plod2 Polyclonal Antibody (CAB6946) is a valuable tool for researchers studying the role of Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 2 (Plod2) in collagen biosynthesis and connective tissue disorders. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting Plod2 protein in human samples, making it ideal for use in Western blot applications.Plod2 is a key enzyme involved in collagen post-translational modification, specifically in the hydroxylation of lysine residues. Dysregulation of Plod2 has been implicated in various human diseases, including Ehlers-Danlos syndrome and fibrosis. By targeting Plod2 with this polyclonal antibody, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.
With its ability to specifically bind to Plod2, the CAB6946 antibody enables researchers to accurately study the expression and function of this enzyme in different cell types and tissues. By understanding the role of Plod2 in collagen metabolism, researchers can further elucidate its implications in physiological and pathological conditions, paving the way for the development of novel therapies for connective tissue disorders and fibrotic diseases.
Product Name:
PLOD2 Rabbit Polyclonal Antibody
SKU:
CAB6946
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-380 of human PLOD2/LH2 (NP_000926.2).
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5352
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLOD2/LH2 antibody (CAB6946) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
