The PLod2/LH2 Monoclonal Antibody is a powerful tool for researchers studying the PLod2/LH2 protein, which plays a critical role in collagen biosynthesis and extracellular matrix formation. This monoclonal antibody, produced using hybridoma technology, is highly specific and reacts with human samples, making it an excellent choice for applications such as Western blotting and immunohistochemistry.PLod2/LH2 is an important enzyme involved in the post-translational modification of collagen, specifically hydroxylation of lysine residues. Dysregulation of PLod2/LH2 activity has been associated with various pathological conditions, including fibrosis, osteogenesis imperfecta, and cancer.
By using this monoclonal antibody, researchers can investigate the expression levels and localization of PLod2/LH2 in different cell types and tissues, providing valuable insights into its function and potential therapeutic applications.Overall, the PLod2/LH2 Monoclonal Antibody is a valuable tool for research in fields such as cell biology, biochemistry, and pathology, offering reliable and specific detection of the PLod2/LH2 protein for a deeper understanding of collagen metabolism and related diseases.
Product Name:
PLOD2/LH2 Monoclonal Antibody
SKU:
CAB22040
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-380 of human PLOD2/LH2 (NP_000926.2).
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5352
Clone Number:
ARC54359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLOD2/LH2 antibody (CAB22040) at1:30000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
