The PLOD1 Polyclonal Antibody (CAB7919) is a valuable tool for researchers studying PLOD1, a key enzyme involved in collagen biosynthesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting PLOD1 in human samples, making it ideal for use in Western blotting applications. By targeting the PLOD1 enzyme, researchers can investigate its role in collagen production and connective tissue disorders, offering insights into potential therapeutic strategies for conditions such as Ehlers-Danlos Syndrome and fibrosis.
The PLOD1 Polyclonal Antibody is a valuable asset for research in the fields of molecular biology, biochemistry, and connective tissue diseases.
Product Name:
PLOD1 Rabbit Polyclonal Antibody
SKU:
CAB7919
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-295 of human PLOD1 (NP_000293.2).
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5351
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLOD1 antibody (CAB7919) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
