The PLOD1 Polyclonal Antibody (CAB14044) is a valuable tool for researchers studying the Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) protein. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is suitable for use in Western blot applications. By targeting the PLOD1 protein, this antibody enables the detection and analysis of PLOD1 in various cell types, making it ideal for research in biochemistry and extracellular matrix biology.PLOD1 is an essential enzyme involved in collagen biosynthesis and plays a crucial role in the post-translational modification of collagen molecules.
Dysregulation of PLOD1 has been associated with various connective tissue disorders and fibrotic diseases, emphasizing the importance of studying its function and regulation. The PLOD1 Polyclonal Antibody provides researchers with a valuable tool to investigate the role of PLOD1 in health and disease, paving the way for potential therapeutic interventions targeting collagen-related conditions.
Product Name:
PLOD1 Rabbit Polyclonal Antibody
SKU:
CAB14044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_000293.2).
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5351
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse heart, using PLOD1 antibody (CAB14044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
