The PLK4 Polyclonal Antibody (CAB9863) is a specialized tool for researchers studying the PLK4 protein, a key player in cell division and centriole duplication. This antibody, produced in rabbits, is highly specific for human samples and has been extensively validated for use in Western blot applications. By binding to the PLK4 protein, this antibody allows for accurate detection and analysis of its expression levels in various cell types, making it an essential tool for studies in cell biology and cancer research.PLK4, also known as polo-like kinase 4, is crucial for maintaining the proper number of centrioles in cells during division, making it a potential target for therapies aimed at disrupting cancer cell growth.
Understanding the function and regulation of PLK4 is essential for advancing our knowledge of cell division mechanisms and developing new treatments for diseases characterized by abnormal cell proliferation. The PLK4 Polyclonal Antibody is a valuable resource for scientists investigating the role of PLK4 in health and disease.
Product Name:
PLK4 Rabbit Polyclonal Antibody
SKU:
CAB9863
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10733
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLK4 antibody (CAB9863) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
