The PLK3 Polyclonal Antibody (CAB8674) is a valuable tool for research involving PLK3, a key protein involved in cell cycle regulation and DNA damage response. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications including Western blotting.PLK3, also known as Polo-like kinase 3, plays a critical role in DNA damage-induced cell cycle arrest and apoptosis. Its involvement in these processes makes it a potential target for cancer therapy and research into genetic disorders. By targeting PLK3, researchers can better understand its role in cell cycle progression and potentially develop novel therapeutic strategies for cancer treatment.
The PLK3 Polyclonal Antibody enables the detection and analysis of PLK3 protein levels in different cell types, making it a versatile tool for studies in cancer biology, cell cycle regulation, and DNA damage response mechanisms. By uncovering the functions of PLK3, researchers can gain valuable insights into its potential as a target for drug development and personalized cancer treatment approaches.
Product Name:
PLK3 Rabbit Polyclonal Antibody
SKU:
CAB8674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 487-646 of human PLK3 (NP_004064.2).
Cytoplasm, Golgi apparatus, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus.
Calculated MW:
72kDa
Observed MW:
72kDa
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis.
Purification Method:
Affinity purification
Gene ID:
1263
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLK3 antibody (CAB8674) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
