The PLK1 Monoclonal Antibody (CAB22595) is a vital tool for researchers studying the PLK1 protein, a key regulator of cell division and a promising target for cancer therapy. This antibody, produced through hybridoma technology, is highly specific to human PLK1 and is validated for use in immunohistochemistry and flow cytometry applications. It binds specifically to the PLK1 protein, allowing for precise detection and analysis in diverse cell types, making it ideal for investigations in oncology and cell biology.
PLK1, also known as polo-like kinase 1, plays a crucial role in cell cycle progression by regulating mitotic entry, spindle assembly, and cytokinesis. Dysregulation of PLK1 is commonly observed in various cancers, making it an attractive target for anti-cancer drug development. Understanding the function and expression of PLK1 is essential for advancing our knowledge of cancer biology and identifying effective therapeutic strategies targeting this protein.
Product Name:
PLK1 Monoclonal Antibody
SKU:
CAB22595
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 272-495 of human PLK1 (NP_005021.2).
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
Purification Method:
Affinity purification
Gene ID:
5347
Clone Number:
ARC52313
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PLK1 Rabbit mAb (CAB22595) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
