The Plin1 Polyclonal Antibody (CAB16295) is a valuable tool for researchers studying perilipin-1 (Plin1), a protein involved in lipid droplet formation and lipid metabolism. This polyclonal antibody, raised in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.Perilipin-1, also known as lipid droplet-associated protein, plays a crucial role in regulating lipid storage and mobilization, making it a key player in metabolic processes such as obesity, diabetes, and cardiovascular disease.
By targeting Plin1 with this antibody, researchers can better understand its role in lipid metabolism and its implications in metabolic disorders.With its high reactivity and specificity, the Plin1 Polyclonal Antibody (CAB16295) is an essential tool for scientists investigating lipid metabolism, obesity, and related disorders. Its ability to detect and analyze Plin1 in various cell types makes it a valuable resource for studies in both basic science and clinical research settings.
Product Name:
Perilipin A Rabbit Polyclonal Antibody
SKU:
CAB16295
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin A (NP_002657.3).
Sequence:
HYVP LPRL SLME PESE FRDI DNPP AEVE RREA ERRA SGAP SAGP EPAP RLAQ PRRS LRSA QSPG APPG PGLE DEVA TPAA PRPG FPAV PREK PKRR VSDS F
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
PERI; PLIN; FPLD4; Perilipin A
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
cytosol, endoplasmic reticulum, lipid droplet
Calculated MW:
56kDa
Observed MW:
56kDa
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5346
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HepG2, using PerilipinA antibody (CAB16295) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
