The Plekhg5 Antibody (PAC059844) is a polyclonal antibody designed for research involving Plekhg5, a protein involved in autophagy and vesicle trafficking processes. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunofluorescence.Plekhg5 plays a crucial role in maintaining cellular homeostasis by regulating autophagosome formation and cargo delivery, making it a key player in cellular quality control mechanisms.
Dysregulation of Plekhg5 has been implicated in various diseases, including neurodegenerative disorders and cancer. Researchers studying autophagy, vesicle trafficking, and related pathways will find the Plekhg5 Antibody to be a valuable tool for detecting and analyzing Plekhg5 protein levels in different cell types and tissues. With its high specificity and sensitivity, this antibody is essential for furthering our understanding of Plekhg5's biological functions and potential implications in disease states.
Antibody Name:
PLEKHG5 Antibody (PACO59844)
Antibody SKU:
PACO59844
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Pleckstrin homology domain-containing family G member 5 protein (821-957AA)
Immunofluorescence staining of PC-3 cells with PACO59844 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Guanine nucleotide exchange factor that activates RHOA and maybe the NF-kappa-B signaling pathway. Involved in the control of neuronal cell differentiation. Plays a role in angiogenesis through regulation of endothelial cells chemotaxis.
Synonyms:
Pleckstrin homology domain-containing family G member 5 (PH domain-containing family G member 5) (Guanine nucleotide exchange factor 720) (GEF720), PLEKHG5, KIAA0720
UniProt Protein Function:
PLEKHG5: Activates the NF-kappa-B signaling pathway and RHOA. Appears to be involved in the control of neuronal cell differentiation. Defects in PLEKHG5 are the cause of distal spinal muscular atrophy autosomal recessive type 4 (DSMA4). Distal spinal muscular atrophy, also known as distal hereditary motor neuronopathy, represents a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. DSMA4 is characterized by childhood onset, generalized muscle weakness and atrophy with denervation and normal sensation. Bulbar symptoms and pyramidal signs are absent. 5 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 1p36.31Cellular Component: cytoplasm; cytosol; endocytic vesicle; intercellular junction; lamellipodiumMolecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity; signal transducer activityBiological Process: positive regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of small GTPase mediated signal transductionDisease: Charcot-marie-tooth Disease, Recessive Intermediate C; Spinal Muscular Atrophy, Distal, Autosomal Recessive, 4
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein that activates the nuclear factor kappa B (NFKB1) signaling pathway. Mutations in this gene are associated with autosomal recessive distal spinal muscular atrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
