PLCZ1 Antibody (PACO37258)
- SKU:
- PACO37258
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
PLCZ1 Antibody (PACO37258)
The PLCZ1 Polyclonal Antibody (PAC037258) is a valuable tool for researchers studying PLCZ1, a crucial enzyme involved in egg activation during fertilization. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot experiments. By binding to the PLCZ1 protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential asset for studies in reproductive biology and fertility research.PLCZ1, also known as phospholipase C zeta 1, plays a pivotal role in the process of sperm-induced egg activation, triggering the events necessary for successful fertilization.
Its involvement in this key reproductive event makes PLCZ1 a promising target for investigating infertility issues and developing assisted reproductive technologies. Understanding the function of PLCZ1 is crucial for advancing our knowledge of reproductive biology and potentially improving fertility treatments for couples struggling with conception.
| Antibody Name: | PLCZ1 Antibody (PACO37258) |
| Antibody SKU: | PACO37258 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 protein (1-415AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: PLCZ1 antibody at 3µg/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse kidney tissue. Lane 3: Human placenta tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 71, 48, 58 kDa. Observed band size: 71 kDa. |
| Background: | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. |
| Synonyms: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC 3.1.4.11) (Phosphoinositide phospholipase C-zeta-1) (Phospholipase C-zeta-1) (PLC-zeta-1) (Testis-development protein NYD-SP27), PLCZ1 |
| UniProt Protein Function: | PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; PhospholipaseChromosomal Location of Human Ortholog: 12p12.3Cellular Component: perinuclear region of cytoplasm; nucleus; cytosolMolecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activityBiological Process: inositol phosphate metabolic process; multicellular organismal development; calcium ion transport; egg activation; lipid catabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| UniProt Code: | Q86YW0 |
| NCBI GenInfo Identifier: | 74714209 |
| NCBI Gene ID: | 89869 |
| NCBI Accession: | Q86YW0.1 |
| UniProt Secondary Accession: | Q86YW0,Q08AQ7, Q96J70 |
| UniProt Related Accession: | Q86YW0 |
| Molecular Weight: | 57,804 Da |
| NCBI Full Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 |
| NCBI Synonym Full Names: | phospholipase C, zeta 1 |
| NCBI Official Symbol: | PLCZ1Â Â |
| NCBI Official Synonym Symbols: | PLCzeta; NYD-SP27; PLC-zeta-1Â Â |
| NCBI Protein Information: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; PI-phospholipase C zeta 1; testis-development related NYD-SP27; phosphoinositide phospholipase C-zeta-1 |
| UniProt Protein Name: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 |
| UniProt Synonym Protein Names: | Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-1; PLC-zeta-1; Testis-development protein NYD-SP27 |
| Protein Family: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase |
| UniProt Gene Name: | PLCZ1Â Â |
| UniProt Entry Name: | PLCZ1_HUMAN |
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