The PLCG1 Polyclonal Antibody (CAB16953) is a valuable tool for researchers studying the PLCG1 protein, an essential enzyme involved in signaling pathways related to cellular growth and differentiation. This antibody, produced in rabbits, is highly specific for detecting PLCG1 in human samples and has been validated for use in Western blot applications.PLCG1 is a key player in intracellular signaling cascades, particularly in pathways activated by growth factors, cytokines, and immune receptors. Its dysregulation has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
By targeting PLCG1 with this antibody, researchers can gain insights into its role in these disease processes and potentially develop novel therapies targeting this enzyme.Overall, the PLCG1 Polyclonal Antibody is a crucial tool for investigating the intricate signaling mechanisms involving PLCG1 and its implications in disease pathogenesis. Researchers in the fields of cell biology, cancer research, and immunology will find this antibody valuable for their studies.
Product Name:
PLC gamma 1 (PLCG1) Rabbit Polyclonal Antibody
SKU:
CAB16953
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PLC gamma 1 (PLCG1) (NP_002651.2).
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1)) Rabbit pAb (CAB16953) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
