The PLCG1 Rabbit Monoclonal Antibody (CAB8899) is a valuable tool for researchers studying the PLCG1 protein, which plays a crucial role in various signaling pathways involved in cell growth, differentiation, and immune response. This high-quality antibody, generated in rabbits, is highly specific and sensitive for detecting PLCG1 in human samples, making it an excellent choice for immunohistochemistry and Western blot applications.PLCG1 is a key enzyme that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), leading to the activation of downstream signaling cascades.
Dysregulation of PLCG1 has been implicated in various diseases, including cancer, neurological disorders, and immune-related conditions.By using the PLCG1 Rabbit Monoclonal Antibody (CAB8899), researchers can gain valuable insights into the function and regulation of PLCG1 in physiological and pathological processes. Its specificity and reliability make it an essential tool for studies aiming to elucidate the role of PLCG1 in health and disease.
Product Name:
PLC gamma 1 (PLCG1) Rabbit Monoclonal Antibody
SKU:
CAB8899
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 710-832 of human PLC gamma 1 (PLC gamma 1 (PLCG1)) (P19174).
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5335
Clone Number:
ARC1345
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1)) Rabbit mAb (CAB8899) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
