The PLCG1 Polyclonal Antibody (CAB7711) is a valuable tool for researchers studying the PLCG1 protein, a key regulator of intracellular signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to PLCG1, this antibody enables researchers to detect and analyze the protein in various cell types, making it an essential component for studies in cell signaling, molecular biology, and cancer research.PLCG1 is a critical enzyme involved in the transduction of extracellular signals into cellular responses, playing a crucial role in cell growth, differentiation, and apoptosis.
Dysregulation of PLCG1 has been implicated in various diseases, including cancer, autoimmune disorders, and cardiovascular conditions. Understanding the function and activity of PLCG1 is essential for developing targeted therapies and treatments for these diseases, making the PLCG1 Polyclonal Antibody a valuable tool for researchers in the field of molecular biology and biomedical sciences.
Product Name:
PLC gamma 1 (PLCG1) Rabbit Polyclonal Antibody
SKU:
CAB7711
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PLC gamma 1 (PLCG1) (NP_002651.2).
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1)) antibody (CAB7711) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
