The Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody is a valuable tool for research involving PLAP, an enzyme that is highly expressed in placental tissue and can also be found in germ cell tumors. This antibody, created using rabbit immunization techniques, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PLAP enzyme, researchers can easily detect and analyze its expression in a variety of cell types.PLAP is a marker often used in diagnosing certain types of cancer, such as seminomas and ovarian germ cell tumors. Its unique presence in placental tissue and tumors makes it a versatile target for studies in oncology and reproductive biology.
By understanding the role of PLAP in these contexts, researchers can gain valuable insights into disease mechanisms and potentially develop targeted therapies for related conditions.Overall, the PLAP Polyclonal Antibody offers a reliable tool for investigating PLAP expression and function in various research settings, from cancer biology to reproductive health. Its high specificity and sensitivity make it a valuable asset for scientists striving to deepen their understanding of PLAP-related diseases and pathways.
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
Purification Method:
Affinity purification
Gene ID:
250
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Placental alkaline phosphatase (PLAP) Rabbit pAb (CAB21424) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
