The PLA2G4F Polyclonal Antibody (PAC063107) is a valuable tool for researchers studying the phospholipase A2 group IVF (PLA2G4F) protein. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the PLA2G4F protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of biochemistry and cell biology.PLA2G4F is an enzyme that plays a crucial role in lipid metabolism, specifically in the hydrolysis of phospholipids to release fatty acids and lysophospholipids. Dysregulation of this enzyme has been linked to various diseases, including cancer, inflammatory disorders, and neurodegenerative conditions.
Therefore, studying the function and expression of PLA2G4F is essential for gaining insights into these pathological processes and potential therapeutic targets.By utilizing the PAC063107 antibody, researchers can delve deeper into the mechanisms underlying PLA2G4F activity and its implications in disease development. This antibody provides a reliable tool for investigating the role of PLA2G4F in various cellular processes, paving the way for advancements in drug discovery and personalized medicine.
Antibody Name:
PLA2G4F Antibody (PACO63107)
Antibody SKU:
PACO63107
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Cytosolic phospholipase A2 zeta protein (74-299AA)
IHC image of PACO63107 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine (By similarity).
PLA2G4F: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. Has a much higher lysophospholipase and PLA(2) activity than PLA2G4B. Potently inhibited by pyrrolidine-2 and Wyeth-1. Its specific activity, inhibitor sensitivity, and low micromolar calcium dependence are similar to PLA2G4A. Translocates to ruffles and dynamic vesicular structures in a calcium-dependent manner. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ Three isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - arachidonic acid; EC 3.1.1.4; PhospholipaseChromosomal Location of Human Ortholog: 15q15.1Cellular Component: cytosolMolecular Function: lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activityBiological Process: phospholipid metabolic process
