PLA2G4E Antibody (PACO63419)
- SKU:
- PACO63419
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | PLA2G4E Antibody (PACO63419) |
| Antibody SKU: | PACO63419 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cytosolic phospholipase A2 epsilon protein (604-868AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63419 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO63419 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. |
| Synonyms: | Cytosolic phospholipase A2 epsilon (cPLA2-epsilon) (EC 3.1.1.4) (Phospholipase A2 group IVE), PLA2G4E |
| UniProt Protein Function: | PLA2G4E: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Translocates to lysosomal membranes in a calcium-dependent fashion. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Two isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4Chromosomal Location of Human Ortholog: 15q15.1Cellular Component: lysosomal membrane; cytosolMolecular Function: phospholipase A2 activity; metal ion bindingBiological Process: phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q3MJ16 |
| NCBI GenInfo Identifier: | 331284217 |
| NCBI Gene ID: | 123745 |
| NCBI Accession: | NP_001193599.1 |
| UniProt Secondary Accession: | Q3MJ16,Q6ZSC0 |
| UniProt Related Accession: | Q3MJ16 |
| Molecular Weight: | 56,715 Da |
| NCBI Full Name: | cytosolic phospholipase A2 epsilon |
| NCBI Synonym Full Names: | phospholipase A2, group IVE |
| NCBI Official Symbol: | PLA2G4EÂ Â |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | cytosolic phospholipase A2 epsilon; cPLA2-epsilon |
| UniProt Protein Name: | Cytosolic phospholipase A2 epsilon |
| UniProt Synonym Protein Names: | Phospholipase A2 group IVE |
| Protein Family: | Cytosolic phospholipase |
| UniProt Gene Name: | PLA2G4EÂ Â |
| UniProt Entry Name: | PA24E_HUMAN |
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