PLA2G4E Antibody (PACO63419)
- SKU:
- PACO63419
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
PLA2G4E Antibody (PACO63419)
The PLA2G4E Polyclonal Antibody (PAC063419) offered by Assay Genie is a valuable tool for researchers studying the PLA2G4E enzyme. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in applications such as Western blotting.PLA2G4E, also known as cytosolic phospholipase A2 epsilon, is involved in the metabolism of phospholipids and plays a role in inflammatory pathways. Research suggests that dysregulation of PLA2G4E may contribute to inflammatory conditions, making it a promising target for therapeutic interventions.
The PAC063419 antibody binds specifically to the PLA2G4E protein, allowing for its detection and analysis in various cell types.With its high specificity and validated performance, the PAC063419 antibody is an essential tool for researchers investigating the function and regulation of PLA2G4E in health and disease. By understanding the role of PLA2G4E in inflammatory processes, researchers can potentially develop new strategies for treating conditions associated with dysregulated inflammation.
| Antibody Name: | PLA2G4E Antibody (PACO63419) |
| Antibody SKU: | PACO63419 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cytosolic phospholipase A2 epsilon protein (604-868AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63419 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO63419 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. |
| Synonyms: | Cytosolic phospholipase A2 epsilon (cPLA2-epsilon) (EC 3.1.1.4) (Phospholipase A2 group IVE), PLA2G4E |
| UniProt Protein Function: | PLA2G4E: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Translocates to lysosomal membranes in a calcium-dependent fashion. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Two isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4Chromosomal Location of Human Ortholog: 15q15.1Cellular Component: lysosomal membrane; cytosolMolecular Function: phospholipase A2 activity; metal ion bindingBiological Process: phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q3MJ16 |
| NCBI GenInfo Identifier: | 331284217 |
| NCBI Gene ID: | 123745 |
| NCBI Accession: | NP_001193599.1 |
| UniProt Secondary Accession: | Q3MJ16,Q6ZSC0 |
| UniProt Related Accession: | Q3MJ16 |
| Molecular Weight: | 56,715 Da |
| NCBI Full Name: | cytosolic phospholipase A2 epsilon |
| NCBI Synonym Full Names: | phospholipase A2, group IVE |
| NCBI Official Symbol: | PLA2G4EÂ Â |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | cytosolic phospholipase A2 epsilon; cPLA2-epsilon |
| UniProt Protein Name: | Cytosolic phospholipase A2 epsilon |
| UniProt Synonym Protein Names: | Phospholipase A2 group IVE |
| Protein Family: | Cytosolic phospholipase |
| UniProt Gene Name: | PLA2G4EÂ Â |
| UniProt Entry Name: | PA24E_HUMAN |
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