PLA2G4B Antibody (PACO62959)
- SKU:
- PACO62959
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
PLA2G4B Antibody (PACO62959)
The PLA2G4B Polyclonal Antibody (PAC062959) is a valuable tool for researchers studying PLA2G4B, an enzyme involved in the metabolism of membrane phospholipids and the production of lipid mediators such as prostaglandins and leukotrienes. This antibody, raised in rabbits, exhibits high specificity for detection of PLA2G4B in human samples, making it ideal for use in Western blot applications.PLA2G4B, also known as cytosolic phospholipase A2 beta, plays a crucial role in the inflammatory response by regulating the release of arachidonic acid and subsequent synthesis of pro-inflammatory lipid mediators.
Dysregulation of PLA2G4B activity has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. Therefore, studying the expression and function of PLA2G4B is essential for understanding the underlying mechanisms of these diseases and developing targeted therapies.Overall, the PAC062959 antibody provides researchers with a reliable tool for investigating the role of PLA2G4B in health and disease, offering valuable insights into potential therapeutic strategies for a range of medical conditions.
| Antibody Name: | PLA2G4B Antibody (PACO62959) |
| Antibody SKU: | PACO62959 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cytosolic phospholipase A2 β protein (514-781AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate. All lanes: P0C869 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 101, 55, 115 kDa. Observed band size: 88 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO62959 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO62959 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine. |
| Synonyms: | Cytosolic phospholipase A2 β (cPLA2-beta) (EC 3.1.1.4) (Phospholipase A2 group IVB), PLA2G4B |
| UniProt Protein Function: | PLA2G4B: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Not inhibited by pyrrolidine-2 and Wyeth-1. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 translocates to membrane vesicles in a calcium-dependent fashion and has activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine.Protein type: Phospholipase; EC 3.1.1.4; Lipid Metabolism - arachidonic acidChromosomal Location of Human Ortholog: 15q11.2-q21.3Cellular Component: cytosol; extracellular region; mitochondrial inner membraneMolecular Function: calcium-dependent phospholipase A2 activity; lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activityBiological Process: glycerophospholipid catabolic process; phosphatidic acid biosynthetic process; phospholipid metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P0C869 |
| NCBI GenInfo Identifier: | 300669659 |
| NCBI Gene ID: | 100137049 |
| NCBI Accession: | P0C869.2 |
| UniProt Secondary Accession: | P0C869,O95712, Q19KD5, Q19KD6, Q59GF9, Q8TB10, Q9UKV7 B4DRT9 |
| UniProt Related Accession: | P0C869 |
| Molecular Weight: | 114,121 Da |
| NCBI Full Name: | Cytosolic phospholipase A2 beta |
| NCBI Synonym Full Names: | phospholipase A2 group IVB |
| NCBI Official Symbol: | PLA2G4BÂ Â |
| NCBI Official Synonym Symbols: | HsT16992; cPLA2-beta  |
| NCBI Protein Information: | cytosolic phospholipase A2 beta |
| UniProt Protein Name: | Cytosolic phospholipase A2 beta |
| UniProt Synonym Protein Names: | Phospholipase A2 group IVB |
| Protein Family: | Cytosolic phospholipase |
| UniProt Gene Name: | PLA2G4BÂ Â |
| UniProt Entry Name: | PA24B_HUMAN |
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