The PLA2G4B Monoclonal Antibody (PAC019501) is a powerful tool for researchers studying the PLA2G4B protein, which is involved in the regulation of inflammation and immune responses. This antibody, produced using cutting-edge technology, specifically targets the PLA2G4B protein in various cell types and is optimized for use in Western blot applications.PLA2G4B, also known as cytosolic phospholipase A2 beta, plays a key role in mediating the release of arachidonic acid, a precursor of inflammatory mediators, from membrane phospholipids. Dysregulation of PLA2G4B has been implicated in various inflammatory diseases, making it a promising target for therapeutic interventions.
By using the PAC019501 antibody, researchers can gain valuable insights into the function of PLA2G4B in the context of immune responses and inflammatory pathways.With its high specificity and sensitivity, the PAC019501 antibody is a valuable tool for studies in immunology, inflammation, and related fields. Researchers can rely on this antibody to accurately detect and analyze PLA2G4B expression, enabling further exploration of its role in health and disease.
Antibody Name:
PLA2G4B Antibody (PACO19501)
Antibody SKU:
PACO19501
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human PLA2G4B
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Mouse stomach tissue, 293T cells, Jurkat cells, Primary antibody: PACO19501(PLA2G4B Antibody) at dilution 1/1400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
Background:
This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acid, . This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.
Synonyms:
phospholipase A2, group IVB (cytosolic)
UniProt Protein Function:
PLA2G4B: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Not inhibited by pyrrolidine-2 and Wyeth-1. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 translocates to membrane vesicles in a calcium-dependent fashion and has activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine.Protein type: Phospholipase; EC 3.1.1.4; Lipid Metabolism - arachidonic acidChromosomal Location of Human Ortholog: 15q11.2-q21.3Cellular Component: cytosol; extracellular region; mitochondrial inner membraneMolecular Function: calcium-dependent phospholipase A2 activity; lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activityBiological Process: glycerophospholipid catabolic process; phosphatidic acid biosynthetic process; phospholipid metabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008]
