The PLA2G2A Polyclonal Antibody (CAB1234) is a valuable tool for researchers studying PLA2G2A, a key enzyme involved in the regulation of inflammatory responses and lipid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot analysis.PLA2G2A, also known as phospholipase A2, group IIA, is known for its role in catalyzing the hydrolysis of phospholipids to release fatty acids and lysophospholipids, leading to the production of inflammatory mediators. Its involvement in lipid signaling pathways and inflammatory processes makes it a target of interest in research related to a variety of diseases, including cardiovascular disorders, neuroinflammation, and cancer.
By enabling the detection and analysis of PLA2G2A in different cell types, the PLA2G2A Polyclonal Antibody (CAB1234) is a valuable tool for investigations in lipid metabolism, inflammation, and related pathways. Understanding the function and regulation of PLA2G2A may provide insights into potential therapeutic strategies for diseases associated with dysregulated lipid metabolism and inflammation.
Product Name:
PLA2G2A Rabbit Polyclonal Antibody
SKU:
CAB1234
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-144 of human PLA2G2A (NP_001155201.1).
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5320
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PLA2G2A antibody (CAB1234) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
