The PKN1 Polyclonal Antibody (CAB0553) is a valuable tool for researchers studying PKN1, a protein kinase involved in various cellular processes such as cell proliferation, apoptosis, and cytoskeletal organization. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PKN1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component in studies related to cell signaling and cancer research.PKN1, also known as protein kinase N1, plays a crucial role in regulating cell growth and survival, making it a potential target for therapeutic interventions in cancer and other diseases.
Understanding the function and activity of PKN1 can provide valuable insights into the mechanisms underlying tumorigenesis and may lead to the development of targeted treatment strategies for cancer patients. By utilizing the PKN1 Polyclonal Antibody, researchers can further explore the role of this protein kinase in cellular signaling pathways and potentially uncover new therapeutic opportunities for combating disease.
Product Name:
PKN1 Rabbit Polyclonal Antibody
SKU:
CAB0553
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3).
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5585
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PKN1 antibody (CAB0553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
