The PKM2 Specific Monoclonal Antibody (CAB22408) is a highly specific antibody designed for research involving pyruvate kinase M2 (PKM2), a key enzyme in the glycolytic pathway that is often upregulated in cancer cells. The monoclonal antibody, generated using hybridoma technology, shows high affinity and specificity for PKM2 in human samples and is validated for use in various applications, including Western blotting and immunohistochemistry.PKM2 is a critical regulator of cancer metabolism, promoting the Warburg effect by driving increased glucose consumption and lactate production in tumor cells. Its overexpression is associated with tumor growth, metastasis, and resistance to therapy, making it a promising target for cancer treatment.
The PKM2 Specific Monoclonal Antibody enables researchers to detect and analyze PKM2 expression levels in cancer cells, providing valuable insights into the role of this enzyme in tumorigenesis and potential therapeutic strategies targeting PKM2.This antibody is a valuable tool for studies in cancer biology, metabolism, and drug discovery, offering researchers the ability to investigate the molecular mechanisms underlying PKM2 dysregulation in cancer and explore its potential as a biomarker or therapeutic target. By understanding the role of PKM2 in cancer progression, researchers can develop more effective strategies for diagnosing and treating cancer.
Product Name:
PKM2-specific Monoclonal Antibody
SKU:
CAB22408
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 380-480 of human PKM2-specific (NP_002645.3).
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
5315
Clone Number:
ARC50917
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PKM2-specific antibody (CAB22408) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
