The PKM Polyclonal Antibody (CAB13905) is a key tool for researchers studying the pyruvate kinase M2 (PKM2) protein, which plays a crucial role in cellular metabolism and energy production. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards PKM and is validated for use in various applications, including Western blotting.PKM2 is an isoform of pyruvate kinase that is often overexpressed in cancer cells, promoting aerobic glycolysis to support rapid cell proliferation. By targeting PKM2 with this antibody, researchers can investigate its role in cancer growth and explore potential therapeutic strategies to disrupt this metabolic pathway.
With its ability to specifically bind to and detect PKM2 in a variety of samples, the PKM Polyclonal Antibody is essential for advancing research in cancer biology, metabolism, and potential drug development targeting PKM2. Gain valuable insights into cellular metabolism and cancer progression with this highly reliable research tool.
Product Name:
PKM2 Rabbit Polyclonal Antibody
SKU:
CAB13905
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of PKM1/2 (NP_001193728.1).
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
5315
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PKM1/2 Rabbit pAb (CAB13905) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
