The PKLR Polyclonal Antibody (CAB12084) is a valuable tool for researchers studying the pyruvate kinase L/R (PKLR) protein, an enzyme involved in glycolysis. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the PKLR protein, this antibody enables researchers to detect and analyze PKLR expression in various cell types.PKLR is a key player in the glycolytic pathway, with mutations in the PKLR gene being associated with disorders such as pyruvate kinase deficiency and hemolytic anemia.
By studying the expression and function of PKLR, researchers can gain insights into metabolic processes and potential therapeutic targets related to these conditions. The PKLR Polyclonal Antibody is therefore a valuable tool for investigating the role of PKLR in normal physiology and disease states, making it ideal for studies in metabolic disorders and related research fields.
Product Name:
PKLR Rabbit Polyclonal Antibody
SKU:
CAB12084
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 446-556 of human PKLR (NP_000289.1).
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5313
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PKLR Rabbit pAb (CAB12084) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 50s.
