The PKA R2 Rabbit Monoclonal Antibody (CAB3889) is a cutting-edge tool for researchers studying the protein kinase A regulatory subunit 2 (PKA R2). This antibody, developed using rabbit monoclonal technology, offers high specificity and sensitivity when detecting PKA R2 in human samples. Validated for use in Western blot applications, this antibody binds specifically to PKA R2, allowing for accurate detection and analysis in a variety of cell types.PKA R2 is a crucial component of the PKA holoenzyme, which plays a central role in cellular signaling pathways and the regulation of various cellular processes. Dysregulation of PKA signaling has been implicated in a wide range of diseases, including cancer, cardiovascular disorders, and neurological conditions.
By studying the expression and activity of PKA R2, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.This PKA R2 Rabbit Monoclonal Antibody is a valuable tool for researchers in the fields of biochemistry, cell biology, and pharmacology who are investigating PKA signaling pathways and their impact on human health. Its high specificity and sensitivity make it an ideal choice for experiments aiming to elucidate the role of PKA R2 in normal cellular function and disease pathology.
Product Name:
PKA RIIα (PRKAR2A) Rabbit Monoclonal Antibody
SKU:
CAB3889
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 312-404 of human PKA RIIα (PRKAR2A) (P13861).
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Purification Method:
Affinity purification
Gene ID:
5576
Clone Number:
ARC0860
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A) Rabbit mAb (CAB3889) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
