The PIRT Monoclonal Antibody (PAC060925) is a valuable tool for researchers studying the PIRT protein, a key player in pain perception and modulation. This antibody, produced in mice, is highly specific for detecting PIRT in human samples and is validated for use in immunofluorescence and immunohistochemistry applications. By binding to the PIRT protein, this antibody allows for precise visualization and analysis of PIRT expression in various tissues and cell types, making it essential for investigations into pain mechanisms and sensory processing.PIRT, also known as phosphoinositide-interacting regulator of transient receptor potential channels, is a crucial regulator of pain sensitivity and is involved in the modulation of TRPV1 and TRPV3 channels.
Understanding the function of PIRT is essential for uncovering novel targets for pain management and developing new therapeutic approaches for chronic pain conditions. Research using the PIRT antibody can provide valuable insights into the role of PIRT in pain signaling pathways and pave the way for the development of innovative pain relief strategies.
IHC image of PACO60925 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO60925 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO60925 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI) (By similarity).
Synonyms:
Phosphoinositide-interacting protein, PIRT
UniProt Protein Function:
PIRT: Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5- bisphosphate (PIP2), but not phosphatidylinositol (PI).Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 17p12Cellular Component: integral to membrane
