PIN1 Polyclonal Antibody (CAB20718)
- SKU:
- CAB20718
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant - Arabidopsis
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | PIN1 Polyclonal Antibody |
| SKU: | CAB20718 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Arabidopsis thaliana |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 391-480 of arabidopsis thaliana PIN1 (NP_177500.1). |
| Sequence: | HADY STAT NDHQ KDVK ISVP QGNS NDNQ YVER EEFS FGNK DDDS KVLA TDGG NNIS NKTT QAKV MPPT SVMT RLIL IMVW RKLI RNPN SY |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | ARABIDOPSIS THALIANA PIN-FORMED 1; ATPIN1; F6D5.2; F6D5_2; PIN-FORMED 1; PIN1 |
| Positive Sample: | Inflorescences |
| Conjugate: | Unconjugated |
| Cellular Localization: | basal plasma membrane, cytoplasm, endoplasmic reticulum, plant-type cell wall, plasma membrane, plasmodesma |
| Calculated MW: | 67kDa |
| Observed MW: | 67kDa |
Encodes an auxin efflux carrier involved in shoot and root development. It is involved in the maintenance of embryonic auxin gradients. Loss of function severely affects organ initiation, pin1 mutants are characterised by an inflorescence meristem that does not initiate any flowers, resulting in the formation of a naked inflorescence stem. PIN1 is involved in the determination of leaf shape by actively promoting development of leaf margin serrations. In roots, the protein mainly resides at the basal end of the vascular cells, but weak signals can be detected in the epidermis and the cortex. Expression levels and polarity of this auxin efflux carrier change during primordium development suggesting that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. PIN1 action on plant development does not strictly require function of PGP1 and PGP19 proteins.
| Purification Method: | Affinity purification |
| Gene ID: | 843693 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Inflorescences, using PIN1 Rabbit pAb (CAB20718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
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