The Pin1 Polyclonal Antibody (CAB10006) from AssayGenie is a highly specific antibody designed for research involving Pin1, a key regulator of cell cycle progression and cellular signaling. This antibody, raised in rabbits, is validated for use in various applications, including Western blot and immunohistochemistry.Pin1 is a unique enzyme that catalyzes the isomerization of specific phosphorylated Ser/Thr-Pro motifs in target proteins, allowing for precise regulation of protein function and stability. Dysfunction of Pin1 has been implicated in various diseases, including cancer, Alzheimer's disease, and heart disease.
By targeting Pin1, researchers can investigate its role in cellular processes such as cell division, DNA damage response, and apoptosis. This antibody enables detection and analysis of Pin1 expression in different cell types and tissues, making it an invaluable tool for studies in cell biology, cancer research, and drug development.Overall, the Pin1 Polyclonal Antibody is a reliable reagent for researchers seeking to unravel the complex functions of Pin1 and its potential as a therapeutic target in disease treatment.
Product Name:
PIN1 Polyclonal Antibody
SKU:
CAB20718
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 391-480 of arabidopsis thaliana PIN1 (NP_177500.1).
Encodes an auxin efflux carrier involved in shoot and root development. It is involved in the maintenance of embryonic auxin gradients. Loss of function severely affects organ initiation, pin1 mutants are characterised by an inflorescence meristem that does not initiate any flowers, resulting in the formation of a naked inflorescence stem. PIN1 is involved in the determination of leaf shape by actively promoting development of leaf margin serrations. In roots, the protein mainly resides at the basal end of the vascular cells, but weak signals can be detected in the epidermis and the cortex. Expression levels and polarity of this auxin efflux carrier change during primordium development suggesting that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. PIN1 action on plant development does not strictly require function of PGP1 and PGP19 proteins.
Purification Method:
Affinity purification
Gene ID:
843693
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Inflorescences, using PIN1 Rabbit pAb (CAB20718) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
