The PIM1 Rabbit Monoclonal Antibody (CAB19695) is a highly specific research tool designed for the detection of the PIM1 protein, a serine/threonine kinase involved in cell growth and survival pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.PIM1 is a critical player in signaling pathways that promote cell proliferation and inhibit apoptosis, making it a potential target for cancer therapy. Its aberrant expression has been linked to various types of cancer, making the PIM1 antibody an essential tool for studying the role of this kinase in oncogenesis.
By detecting and analyzing the expression of PIM1 in various cell types and tissues, researchers can better understand the mechanisms by which this protein contributes to tumorigenesis. This antibody is a valuable asset for studies in cancer biology, drug development, and personalized medicine, providing crucial insights into the role of PIM1 in cancer progression and therapeutic resistance.
Product Name:
PIM1 Rabbit Monoclonal Antibody
SKU:
CAB19695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PIM1 (P11309).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
Purification Method:
Affinity purification
Gene ID:
5292
Clone Number:
ARC0175
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 22Rv1 cells, using PIM1 Rabbit mAb (CAB19695) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
