The PIM1 Polyclonal Antibody (CAB14210) is specifically designed for research involving the PIM1 protein, a serine/threonine kinase that plays a key role in various cellular processes, including cell proliferation and survival. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the PIM1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in oncology and cell biology.PIM1 is known to be overexpressed in various cancer types, including leukemia, lymphoma, and solid tumors. Its role in promoting cell growth and survival makes it a promising target for cancer therapy and research.
By studying the activity of PIM1, researchers can gain valuable insights into the mechanisms of cancer development and potentially identify new therapeutic strategies to target this protein in cancer treatment.Overall, the PIM1 Polyclonal Antibody is a valuable tool for researchers interested in studying the role of PIM1 in cancer and other cellular processes. Its high reactivity and specificity make it a reliable choice for Western blot analysis and other research applications. By utilizing this antibody, scientists can further our understanding of PIM1 biology and potentially contribute to the development of novel cancer therapies.
Product Name:
PIM1 Rabbit Polyclonal Antibody
SKU:
CAB14210
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PIM1 (NP_001230115.1).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
Purification Method:
Affinity purification
Gene ID:
5292
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PIM1 Rabbit pAb (CAB14210) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
