PIK3R6 Antibody (PACO60997)
- SKU:
- PACO60997
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
PIK3R6 Antibody (PACO60997)
The PIK3R6 Polyclonal Antibody (PACO60997) is a versatile tool for researchers studying the PI3K/AKT signaling pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By targeting the PIK3R6 protein, this antibody allows for the detection and analysis of PI3K regulatory subunit 6 in various cell types.PIK3R6 is a crucial component of the PI3K/AKT pathway, which plays a pivotal role in cell growth, proliferation, survival, and metabolism. Dysregulation of this pathway is commonly observed in cancer and other diseases, making PIK3R6 an attractive target for therapeutic interventions.
By utilizing the PIK3R6 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of this protein, ultimately advancing our understanding of its role in disease pathogenesis and potential therapeutic strategies.Overall, the PIK3R6 Polyclonal Antibody is an essential tool for researchers interested in unraveling the complexities of the PI3K/AKT signaling pathway and its implications in various disease states. Its reliability and specificity make it a valuable asset for studies in cancer biology, cell signaling, and drug development.
| Antibody Name: | PIK3R6 Antibody (PACO60997) |
| Antibody SKU: | PACO60997 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Phosphoinositide 3-kinase regulatory subunit 6 protein (566-667AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60997 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO60997 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60997 diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Regulatory subunit of the PI3K γ complex. Acts as an adapter to drive activation of PIK3CG by beta-γ G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-γ G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-γ G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K γ complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS. |
| Synonyms: | Phosphoinositide 3-kinase regulatory subunit 6 (Phosphoinositide 3-kinase γ adapter protein of 87 kDa) (p84 PI3K adapter protein) (p84 PIKAP) (p87 PI3K adapter protein) (p87PIKAP), PIK3R6, C17orf38 |
| UniProt Protein Function: | PIK3R6: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS1 bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.Chromosomal Location of Human Ortholog: 17p13.1Cellular Component: cytosol; membrane; phosphoinositide 3-kinase complex; plasma membraneMolecular Function: 1-phosphatidylinositol-3-kinase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein bindingBiological Process: angiogenesis; G-protein coupled receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; platelet activation; positive regulation of angiogenesis; positive regulation of MAP kinase activity; positive regulation of T cell differentiation; regulation of natural killer cell mediated cytotoxicity; regulation of phosphoinositide 3-kinase activity |
| UniProt Protein Details: | |
| NCBI Summary: | Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q5UE93 |
| NCBI GenInfo Identifier: | 58082081 |
| NCBI Gene ID: | 146850 |
| NCBI Accession: | NP_001010855.1 |
| UniProt Secondary Accession: | Q5UE93 |
| UniProt Related Accession: | Q5UE93 |
| Molecular Weight: | |
| NCBI Full Name: | phosphoinositide 3-kinase regulatory subunit 6 isoform 1 |
| NCBI Synonym Full Names: | phosphoinositide-3-kinase regulatory subunit 6 |
| NCBI Official Symbol: | PIK3R6Â Â |
| NCBI Official Synonym Symbols: | C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)Â Â |
| NCBI Protein Information: | phosphoinositide 3-kinase regulatory subunit 6 |
| UniProt Protein Name: | Phosphoinositide 3-kinase regulatory subunit 6 |
| UniProt Synonym Protein Names: | Phosphoinositide 3-kinase gamma adapter protein of 87 kDa; p84 PI3K adapter protein; p84 PIKAP; p87 PI3K adapter protein; p87PIKAP |
| Protein Family: | |
| UniProt Gene Name: | PIK3R6Â Â |
| UniProt Entry Name: |
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