The PIK3R4 Polyclonal Antibody (CAB15828) is a valuable tool for researchers studying the PIK3R4 gene, which encodes the p150 isoform of phosphoinositide 3-kinase (PI3K). This enzyme is involved in regulating various cellular processes, including cell growth, proliferation, and survival. The antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications.By targeting the PIK3R4 protein, this antibody enables researchers to detect and analyze the expression levels of PI3K in different cell types, providing key insights into its role in cellular signaling pathways.
This antibody is particularly useful for investigations in the fields of cancer research, cell biology, and signal transduction.Given the importance of PI3K in various diseases, including cancer and metabolic disorders, understanding its regulation and activity is crucial for developing targeted therapies. The PIK3R4 Polyclonal Antibody offers researchers a reliable tool for exploring the functions of this critical enzyme and its potential as a therapeutic target.
Product Name:
PIK3R4/VPS15 Rabbit Polyclonal Antibody
SKU:
CAB15828
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4/VPS15 (NP_055417.1).
Cytoplasmic vesicle, Late endosome, Lipid-anchor, Membrane, autophagosome.
Calculated MW:
153kDa
Observed MW:
150kDa
Predicted to enable protein serine/threonine kinase activity. Involved in positive regulation of phosphatidylinositol 3-kinase activity; receptor catabolic process; and regulation of cytokinesis. Located in late endosome and microtubule cytoskeleton.
Purification Method:
Affinity purification
Gene ID:
30849
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PIK3R4/VPS15 antibody (CAB15828) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
