The PIK3R1 Polyclonal Antibody (CAB11526) is a valuable tool for researchers studying the PIK3R1 protein, a critical regulator of the PI3K/AKT signaling pathway. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the PIK3R1 protein, this antibody enables precise detection and analysis in various cell types, facilitating investigations in areas such as cancer biology, cell signaling, and drug development.PIK3R1, also known as phosphoinositide-3-kinase regulatory subunit 1, plays a crucial role in controlling cell growth, proliferation, and survival by modulating the activity of PI3K/AKT signaling.
Dysregulation of this pathway is implicated in various diseases, including cancer, diabetes, and cardiovascular disorders. Therefore, understanding the function and regulation of PIK3R1 is essential for developing targeted therapies and precision medicine approaches in these disease contexts.Overall, the PIK3R1 Polyclonal Antibody (CAB11526) is a reliable tool for researchers aiming to explore the intricate mechanisms of the PI3K/AKT signaling pathway and its impact on cellular physiology and disease progression.
Product Name:
PI3 Kinase p85 alpha Rabbit Polyclonal Antibody
SKU:
CAB11526
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-87MG cells, using PI3 Kinase p85 alpha Rabbit pAb (CAB11526) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
