The PIK3R1 Polyclonal Antibody (CAB11402) is a valuable tool for researchers studying the PIK3R1 protein, a key regulator of the PI3K pathway involved in cell growth, survival, and metabolism. This antibody is produced in rabbits and is specifically reactive with human samples, making it ideal for Western blot applications.PIK3R1, also known as phosphoinositide-3-kinase regulatory subunit 1, is a critical component of the PI3K signaling cascade, which is implicated in cancer, diabetes, and other diseases. By targeting PIK3R1 with this antibody, researchers can detect and analyze the expression and activity of this protein in various cell types, providing insights into its role in disease processes.
Understanding the function of PIK3R1 is essential for developing targeted therapies that manipulate the PI3K pathway in disease states. With the PIK3R1 Polyclonal Antibody, researchers can further explore the molecular mechanisms underlying PIK3R1 signaling and its potential as a therapeutic target in cancer and other disorders.
Product Name:
PI3 Kinase p85 alpha Rabbit Polyclonal Antibody
SKU:
CAB11402
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (CAB11402) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
