The PIK3R1 Polyclonal Antibody (CAB11177) is a valuable tool for researchers studying the PIK3R1 protein, which plays a crucial role in cell signaling pathways related to cell growth, proliferation, and survival. This antibody, produced in rabbits, is highly specific to human samples and is validated for Western blot applications.PIK3R1, also known as p85α, is a regulatory subunit of phosphoinositide 3-kinase (PI3K), a key enzyme involved in intracellular signaling. Aberrant PI3K signaling has been linked to various diseases, including cancer, diabetes, and immune disorders.
By targeting the PIK3R1 protein, researchers can gain insights into the mechanisms underlying these diseases and potentially identify therapeutic targets.With its high reactivity and specificity, the PIK3R1 Polyclonal Antibody is a valuable tool for studying PIK3R1 expression and function in different cell types and disease contexts. It allows for the detection and analysis of PIK3R1 levels, providing crucial information for research in cell biology, molecular biology, and drug development.
Product Name:
PI3 Kinase p85 alpha Polyclonal Antibody
SKU:
CAB11177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852664.1).
cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit pAb (CAB11177) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
