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PI3 Kinase p85 alpha Rabbit Polyclonal Antibody (CAB0054)

SKU:
CAB0054
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-PI3 Kinase p85 alpha Antibody CAB0054
  • Immunology Antibodies 1 Anti-PI3 Kinase p85 alpha Antibody CAB0054
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PI3 Kinase p85 alpha Rabbit Polyclonal Antibody
SKU:CAB0054
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852664.1).
Sequence:LQYL LKHF FKLS QTSS KNLL NARV LSEI FSPM LFRF SAAS SDNT ENLI KVIE ILIS TEWN ERQP APAL PPKP PKPT TVAN NGMN NNMS LQDA EWYW GDIS REEV NEKL RDTA DGTF LVRD ASTK MHGD YTLT LRKG GNNK L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200
Synonyms:p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA; PI3 Kinase p85 alpha
Positive Sample:Jurkat,MCF7
Conjugate:Unconjugated
Cellular Localization:cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:84kDa
Observed MW:85kDa

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Purification Method:Affinity purification
Gene ID:5295
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit pAb (CAB0054) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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