The PIK3R1 Polyclonal Antibody (CAB0054) is a valuable tool for researchers studying the PIK3R1 protein, a key regulator in the PI3K/AKT signaling pathway involved in cell growth, proliferation, and survival. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting human PIK3R1 in Western blot analyses. PIK3R1, also known as p85α, serves as the regulatory subunit of the PI3K complex, playing a crucial role in mediating cellular responses to growth factors, insulin, and other stimuli. Dysregulation of the PI3K/AKT pathway, often driven by mutations in PIK3R1, is frequently observed in cancer and other diseases, making this antibody a valuable tool for investigating potential therapeutic targets.
By enabling precise detection and analysis of PIK3R1 in various cell types, this antibody is ideal for research in molecular biology, cancer biology, and drug development. Understanding the function and dysregulation of PIK3R1 can provide valuable insights into disease mechanisms and potential therapeutic strategies.
Product Name:
PI3 Kinase p85 alpha Rabbit Polyclonal Antibody
SKU:
CAB0054
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852664.1).
cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5295
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit pAb (CAB0054) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
