The PIK3CA Polyclonal Antibody (CAB0265) is specially designed for research involving PIK3CA, a gene that encodes the catalytic subunit of phosphatidylinositol-3-kinase (PI3K). This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PIK3CA protein, researchers can easily detect and analyze its expression in various cell types.PIK3CA is known to play a crucial role in cell growth, survival, and proliferation, making it a key player in cancer development and progression. Mutations in the PIK3CA gene have been linked to various types of cancer, making it a promising target for cancer therapy research.
Understanding the activity of PIK3CA can provide valuable insights into cancer biology and potentially lead to the development of targeted therapies for cancer patients.In conclusion, the PIK3CA Polyclonal Antibody is an essential tool for researchers studying the role of PIK3CA in cancer biology and related fields. Its high reactivity and specificity make it ideal for use in Western blot applications, facilitating accurate detection and analysis of PIK3CA expression in various experimental settings.
Product Name:
PIK3CA Rabbit Polyclonal Antibody
SKU:
CAB0265
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
cytoplasm, cytosol, intercalated disc, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
124kDa
Observed MW:
110kDa
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Purification Method:
Affinity purification
Gene ID:
5290
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PIK3CA Rabbit pAb (CAB0265) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
