The PIK3C3/VPS34 Antibody (CAB12483) is a high-quality antibody developed for reliable detection and analysis of target proteins. This enzyme plays a key role in regulating various cellular processes, including autophagy, endocytosis, and intracellular trafficking.Raised in rabbits, this antibody is highly specific and sensitive for detecting PIK3C3 protein in human samples. It has been validated for use in Western blot applications, allowing for accurate and reliable protein analysis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PIK3C3/VPS34 Antibody
SKU:
CAB12483
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VPS34, Vps34, hVps34, PIK3C3/VPS34
Positive Sample:
293T, C6, Mouse lung, Mouse brain, Rat brain
Cellular Localization:
Cytoplasmic Vesicle, Late Endosome, Midbody, Autophagosome.
Calculated MW:
102kDa
Observed MW:
100kDa
Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody.
Purification Method
Affinity purification
Gene ID
5289
RRID
AB_2759326
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PIK3C3/VPS34 Rabbit pAb (CAB12483) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit pAb (CAB12483) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
