The PIK3C3 Polyclonal Antibody (CAB12483) is a valuable tool for researchers studying the PIK3C3 gene, which encodes the class III phosphatidylinositol 3-kinase enzyme. This enzyme plays a key role in regulating various cellular processes, including autophagy, endocytosis, and intracellular trafficking.Raised in rabbits, this antibody is highly specific and sensitive for detecting PIK3C3 protein in human samples. It has been validated for use in Western blot applications, allowing for accurate and reliable protein analysis.
By targeting the PIK3C3 protein, researchers can investigate its role in cell signaling pathways and its potential implications in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes.The PIK3C3 Polyclonal Antibody is a versatile tool for researchers interested in understanding the functions of the PIK3C3 enzyme and its involvement in various cellular processes. Its high reactivity and specificity make it an ideal choice for studies in molecular biology, cell biology, and disease research.
Product Name:
PIK3C3/VPS34 Rabbit Polyclonal Antibody
SKU:
CAB12483
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-412 of human PIK3C3/VPS34 (NP_002638.2).
Cytoplasmic vesicle, Late endosome, Midbody, autophagosome.
Calculated MW:
102kDa
Observed MW:
100kDa
Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody.
Purification Method:
Affinity purification
Gene ID:
5289
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 antibody (CAB12483) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
